Description:
This protocol is designed to extract and purify DNA of 100 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. Up to 400 mg agarose can be processed per spin column. Simply add the specially formulated Buffer DD to dissolve the gel slice containing your DNA sample. After loading to the column, DNA binds selectively to silicon membrane in the presence of the chaotropic salt guanidine thiocyanate. Bound DNA is purified in a series of rapid wash-and-spin steps to remove contaminants, and then eluted using a low salt solution. This simple method eliminates the need for organic solvent extractions and DNA precipitation, allowing for rapid purification of many samples simultaneously.
