Description:
In the presence of the chaotropic salt guanidine thiocyanate, DNA amplified by PCR binds selectively to silicon membrane pre-packed in the spin columns. Bound DNA is purified in a series of rapid wash-and-spin steps to remove contaminating primers, nucleotides, and salts, and then eluted using a low salt solution. This simple method eliminates the need for organic solvent extractions and DNA precipitation, allowing for rapid purification of many samples simultaneously.
